Can neuron modeling constrained by ultrafast imaging data extract the native function of ion channels?

Filipis L, Canepari M.

Front Comput Neurosci. 2023 May 24;17:1192421. doi: 10.3389/fncom.2023.1192421.

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Nav1.2 and BK channel interaction shapes the action potential in the axon initial segment.

Filipis L, Blömer LA, Montnach J, Loussouarn G, De Waard M, Canepari M.

J Physiol. 2023 May;601(10):1957-1979. doi: 10.1113/JP283801.

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In vivo spatiotemporal control of voltage-gated ion channels by using photoactivatable peptidic toxins.

Montnach J, Blömer LA, Lopez L, Filipis L, Meudal H, Lafoux A, Nicolas S, Chu D, Caumes C, Béroud R, Jopling C, Bosmans F, Huchet C, Landon C, Canepari M, De Waard M.

Nat Commun. 2022 Jan 20;13(1):417. doi: 10.1038/s41467-022-27974-w.

 

Cal-520FF is the Present Optimal Ca2+ Indicator for Ultrafast Ca2+ Imaging and Optical Measurement of Ca2+ Currents.

Blömer LA, Filipis L, Canepari M.

J Fluoresc. 2021 May;31(3):619-623. doi: 10.1007/s10895-021-02701-8.

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Ultrafast Sodium Imaging of the Axon Initial Segment of Neurons in Mouse Brain Slices.

Blömer LA, Canepari M, Filipis L.

Curr Protoc. 2021 Mar;1(3):e64. doi: 10.1002/cpz1.64.

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Optical measurement of physiological sodium currents in the axon initial segment.

Filipis L, Canepari M.

J Physiol. 2021 Jan;599(1):49-66. doi: 10.1113/JP280554. 

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Is Purkinje Neuron Hyperpolarisation Important for Cerebellar Synaptic Plasticity? A Retrospective and Prospective Analysis.

Canepari M.

Cerebellum. 2020 Dec;19(6):869-878. doi: 10.1007/s12311-020-01164-0.

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The origin of physiological local mGluR1 supralinear Ca2+ signals in cerebellar Purkinje neurons.

Ait Ouares K, Canepari M.

J Neurosci. 2020 Feb 26;40(9):1795-1809. doi: 10.1523/JNEUROSCI.2406-19.2020.

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Imaging Native Calcium Currents in Brain Slices.

Ait Ouares K, Jaafari N, Kuczewski N, Canepari M.

Adv Exp Med Biol. 2020;1131:73-91. doi: 10.1007/978-3-030-12457-1_4.

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Editorial: New Insights on Neuron and Astrocyte Function From Cutting-Edge Optical Techniques.

Antic SD, Baker BJ, Canepari M.

Front Cell Neurosci. 2019 Oct 15;13:463. doi: 10.3389/fncel.2019.00463.

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Dopamine and action potential generation in the axon initial segment.

Canepari M.

J Physiol. 2019 Jul;597(13):3251-3252. doi: 10.1113/JP278217

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Two Distinct Sets of Ca2+ and K+ Channels Are Activated at Different Membrane Potentials by the Climbing Fiber Synaptic Potential in Purkinje Neuron Dendrites.

Ait Ouares K, Filipis L, Tzilivaki A, Poirazi P, Canepari M.

J Neurosci. 2019 Mar 13;39(11):1969-1981. doi: 10.1523/JNEUROSCI.2155-18.2018. 

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Synthesis by native chemical ligation and characterization of the scorpion toxin AmmTx3.

Zoukimian C, Meudal H, De Waard S, Ouares KA, Nicolas S, Canepari M, Béroud R, Landon C, De Waard M, Boturyn D.

Bioorg Med Chem. 2019 Jan 1;27(1):247-253. doi: 10.1016/j.bmc.2018.12.009. 

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Opto nongenetics inhibition of neuronal firing.

Ait Ouares K, Beurrier C, Canepari M, Laverne G, Kuczewski N.

Eur J Neurosci. 2019 Jan;49(1):6-26. doi: 10.1111/ejn.14251.

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Two-photon probes for in vivo multicolor microscopy of the structure and signals of brain cells.

Ricard C, Arroyo ED, He CX, Portera-Cailliau C, Lepousez G, Canepari M, Fiole D.

Brain Struct Funct. 2018 May 11. doi: 10.1007/s00429-018-1678-1.

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A novel multisite confocal system for rapid Ca2+ imaging from submicron structures in brain slices.

Filipis L, Ait Ouares K, Moreau P, Tanese D, Zampini V, Latini A, Bleau C, Bleau C, Graham J, Canepari M.

J Biophotonics. 2018 Mar;11(3). doi: 10.1002/jbio.201700197.

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Recent advances in patterned photostimulation for optogenetics.

Ronzitti E, Ventalon C, Canepari M, Forget BC, Papagiakoumou E, Emiliani V.​

J Opt. 2017 Oct 12;19(11): 113001. doi: 10.1088/2040-8986/aa8299.

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Imaging membrane potential changes from dendritic spines using computer-generated holography.

Tanese D, Weng JY, Zampini V, De Sars V, Canepari M, Rozsa B, Emiliani V, Zecevic D.

Neurophotonics. 2017 Jul;4(3):031211. doi: 10.1117/1.NPh.4.3.031211.

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A generalised method to estimate the kinetics of fast Ca2+ currents from Ca2+ imaging experiments.

Ait Ouares K, Jaafari N, Canepari M.

J Neurosci Methods. 2016 May 6;268:66-77. doi: 10.1016/j.jneumeth.2016.05.005.

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Functional coupling of diverse voltage-gated Ca2+ channels underlies high-fidelity of fast dendritic Ca2+ signals during burst firing.

Jaafari N, Canepari M.

J Physiol. 2016 Feb 15;594(4):967-83. doi: 10.1113/JP271830. 

 

Combining Membrane Potential Imaging with Other Optical Techniques.

Jaafari N, Vogt KE, Saggau P, Leslie LM, Zecevic D, Canepari M.

Adv Exp Med Biol. 2015;859:103-25. doi: 10.1007/978-3-319-17641-3_4. Review.

 

Imaging Submillisecond Membrane Potential Changes from Individual Regions of Single Axons, Dendrites and Spines.

Popovic M, Vogt K, Holthoff K, Konnerth A, Salzberg BM, Grinvald A, Antic SD, Canepari M, Zecevic D.

Adv Exp Med Biol. 2015;859:57-101. doi: 10.1007/978-3-319-17641-3_3. Review.

 

Using simultaneous voltage and calcium imaging to study fast Ca2+ currents.

Jaafari N, Marret E, Canepari M.

Neurophotonics 2015 Apr.-June 2(2):021010. doi: 10.1117/1.NPh.2.2.021010

 

Combined optogenetics and voltage sensitive dye imaging at single cell resolution.

Willadt S, Canepari M, Yan P, Loew LM, Vogt KE.

Front Cell Neurosci. 2014 Oct 8;8:311. doi: 10.3389/fncel.2014.00311. eCollection 2014.

 

Imaging fast calcium currents beyond the limitations of electrode techniques.

Jaafari N, De Waard M, Canepari M.

Biophys J. 2014 Sep 16;107(6):1280-8. doi: 10.1016/j.bpj.2014.07.059.

 

Firing of hippocampal neurogliaform cells induces suppression of synaptic inhibition.

Lee G, Stewart R, Canepari M, Capogna M  

J Neurosci. 2014 Jan 22;34(4):1280-92. doi: 10.1523/JNEUROSCI.3046-13.2014.

 

Combining Ca2+ imaging with -glutamate photorelease.

Canepari M, De Waard M, Ogden D.

Cold Spring Harb Protoc. 2013 Dec 1;2013(12). pii: pdb.prot073122. doi: 10.1101/pdb.prot073122.

 

Combining Ca2+ and membrane potential imaging in single neurons.

Canepari M, Vogt KE, De Waard M, Zecevic D.

Cold Spring Harb Protoc. 2013 Dec 1;2013(12). pii: pdb.prot073114. doi: 10.1101/pdb.prot073114.

 

Combining calcium imaging with other optical techniques.

Canepari M, Zecevic D, Vogt KE, Ogden D, De Waard M.

Cold Spring Harb Protoc. 2013 Dec 1;2013(12). pii: pdb.top066167. doi: 10.1101/pdb.top066167.

 

Economic and simple system to combine single-spot photolysis and whole-field fluorescence imaging.

Jaafari N, Henson M, Graham J, Canepari M.

J Biomed Opt. 2013;18(6):60505. doi: 10.1117/1.JBO.18.6.060505.

 

Light sources and cameras for standard in vitro membrane potential and high-speed ion imaging.

Davies R, Graham J, Canepari M.

J Microsc. 2013 Jul;251(1):5-13. doi: 10.1111/jmi.12047. Epub 2013 May 20. Review