Calcium currents are routinely measured in voltage-clamp using the patch clamp technique. We developed an original approach to measure fast calcium currents beyond the limitations of the patch clamp technique. We use this method to investigate the physiological role and function of voltage-gated calcium channels expressed in the dendrites of several types of neurons in the rodent nervous system.

Optical measurements of dentritic calcium currents

research team  voltage calcium neuron imaging channels marco canepari

Fluorescence image of a mouse cerebellar Purkinje neuron.

 

The red and blue traces are the local changes of membrane potential and of intracellular calcium concentration recorded optically

 

The white trace is the membrane potential change associated with a climbing fibre synaptic potential recorded with a patch electrodie.

 

 

Our team is exploring the role and function of voltage-gated calcium channels expressed in neuronal dendrites, but also other channels including sodium and potassium channels. We investigate different neurons in various areas of the brain. 

 

Our research activity is carried on in collaboration with other scientists within the Labex  ICST or outside this consortium.

 

A partial list of our current collaborators includes: