Scheme of microscope in voltage and calcium imaging position

Methods for the physiology community

At the top, calcium (blue) and voltage (red) fluorescence images of the apical dendrite of a mouse hippocampal pyramidal neuron. At the bottom, optical recordings of membrane potential changes and of associated calcium currents.

research team  voltage calcium neuron imaging channels marco canepari
 
The advance of scientific knowledge relies on the continuous development of novel methodologies to perform unprecedented measurements in order to answer fundamental open questions. In our team we constantly work on new approaches to measure local currents and membrane potential changes using the unique technology available in the laboratory. 
 
The innovative aspect of our project is the apparatus (left). The unique system available at the LiPhy permits simultaneous illumination at several different wavelenghts and simultaneous imaging at two emission wavelenghts.
research team  voltage calcium neuron imaging channels marco canepari
 
Our system  allows simultaneous recording of membrane potential changes and of associated calcium currents (right).
 
An original method to record fast calcium currents optically at their site of origin. This novel approach will permit the study of native calcium  channels in situ.